A new adjustment calculus is presented to determine the true intraparticle distribution of bound protein within chromatographic beads from confocal fluorescence slice series. The calculus does not require knowledge about optical properties of different chromatographic materials like refractive index and turbidity. but it depends on a parameter which can be adjusted interactively. The ... https://www.bekindtopets.com/flash-find-Luhta-Henttola-Tops-great-save/