Background: Lyme disease (LD) is emerging in many parts of central and eastern copyright. Serological testing is most commonly used to support laboratory diagnosis of LD. Standard two-tiered testing (STTT) for LD involves detection of Borrelia burgdorferi antibodies using an enzyme immunoassay (EIA) followed by IgM and/or IgG immunoblots. https://www.animationbengal.com/